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. 2009 Dec 3;4(12):e8146. doi: 10.1371/journal.pone.0008146

Figure 5. PI3K and MAPK pathway inhibitors reduced migration and invasion of JCV T-Ag transfected cells.

Figure 5

Migration (panels A and B) and invasion (panels C and D) were performed using Boyden chambers without or with Matrigel. LY294002 (25 µM) and U0126 (10 µM) were added to both upper and lower chambers. Cells that invaded through Matrigel, and migrated through the 8 µm pores in the membrane were stained with DAPI. Cell counting was performed with ImageJ software. Data in bar graphs demonstrate fold changes normalized to vector cells (means±SE) obtained from 3 independent experiments. Significant inhibition in both migration and invasion was observed in both HCT116 and SW837 cells with LY294002 and U0126 individually and in combination.