FIGURE 8.

Association of eNOS with AMPK in endothelial cells. Shown in this figure are the results of an eNOS-AMPK co-immunoprecipitation experiment in BAEC transfected with control, eNOS, or AMPK siRNA targeting constructs. Immunoprecipitations using eNOS polyclonal antiserum were performed, as described under “Experimental Procedures.” Immunoprecipitated complexes were resolved by SDS-PAGE and probed in immunoblots using an antibody against AMPK. Cell lysates were also probed in immunoblots probed with anti-eNOS and anti-AMPK antibodies to verify “knockdown” of target proteins. The results shown here are representative of 4–10 independent experiments that yielded similar results. IP, immunoprecipitate; IB, immunoblot.