FIGURE 4.

A, EP2 receptor gene expression is significantly reduced in pten^−/− cells (closed bars) compared with WT cells (open bars) by quantitative PCR. *, p < 0.0001; **, p < 0.005; #, p < 0.05; compared with WT condition. Error bars indicate ±S.D. B, PI3K blockade with wortmannin (50 nm) up-regulates EP2 receptor gene expression in pten^−/− cells. Results are representative of triplicate studies. *, p < 0.0001. C, EP2 mRNA stability in actinomycin-treated pten^−/− versus WT fibroblasts. Results are pooled from five independently performed experiments. *, p < 0.05.