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. 2009 Aug 25;284(47):32279–32287. doi: 10.1074/jbc.M109.021428

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Stimulation of the β₂AR induces agonist dose-dependent and dynamic receptor phosphorylation by PKA and GRK. A, the β₁β₂AR-KO cardiac myocytes expressing FLAG-β₂ARs were stimulated with different concentrations of Iso and blotted for phosphorylation by PKA (Ser(P)^261/262) or GRK (Ser(P)^355/356). The Western blots in A were quantified and plotted as -fold increases over base-line levels for the PKA-dependent phosphorylation (B) and GRK-dependent phosphorylation (C). D, myocytes expressing FLAG-β₂ARs were stimulated with 10 μm Iso for the indicated time to examine the PKA- and GRK-mediated phosphorylation. E and F, the Western blots in D were quantified and plotted as -fold increases over base-line levels. *, p < 0.05; **, p < 0.01 when compared with the base-line levels by one-way ANOVA. a.u., arbitrary units.