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. 2009 Oct 5;284(47):32296–32304. doi: 10.1074/jbc.M109.050658

FIGURE 4.

FIGURE 4.

Proteolysis of MdfA by PK. A, 3 μg of Cys-less MdfA were incubated in the absence (lane 1) or presence of PK without or with various compounds. At the indicated times, PMSF was added to stop the reaction. CF1 and CF2 designate the cleavage fragments that were observed on Coomassie-stained SDS-PAGE. Time points at 0 and 10 min indicate the fast part of the cleavage kinetics and are shown as controls. B, kinetics of proteolysis of Cys-less MdfA in the absence (lanes 1–7) or presence (lanes 8–14) of TPP. At the indicated times, the reaction was stopped by PMSF. C, proteolysis of MdfA-A147C and mBBr-MdfA-A147C by PK in the absence or presence of TPP or Cm. D, proteolysis of Cys-less MdfA by PK in the absence or presence of TPP, Cm, or free mBBr.