FIGURE 8.

Influence of γ2 and chimeric γ2 carrying the CTD of γ1 on agonist-induced currents of heteromeric GluR1(Q)flip/GluR2(R)flop receptors. Mean glutamate- and kainate-induced responses of GluR1(Q)flip/GluR2(R)flop receptors in coexpression with γ2 and its C-terminal chimera recorded 4 days after injection of cRNA in Xenopus oocytes. Concentrations of the applied agonists were 300 μm glutamate (Glu) and 150 μm kainate (KA). Each bar represents the mean (±S.E.) of 9 or 10 oocytes. Black asterisks show significant differences compared with GluR1(Q)flip/GluR2(R)flop; open circles show differences of chimeric γ2 compared with wildtype γ2 (*** and °°°, p < 0.005; Student's t test). The assembly of heteromeric receptor complexes was verified by analysis of current-voltage relationships taking advantage of the linear current-voltage relationship of receptor complexes containing edited (R) receptor variants.