FIGURE 2.

Effects of MIF (50 and 250 ng/ml) and PD 98058 (25 μm), a specific inhibitor of MEK1/2 phosphorylation, on U373 MG cells. Cell proliferation (A, n = 10) was evaluated after 48 h using the MTT assay. Migration (B, n = 7) and invasion (C, n = 5) were assessed using the modified Boyden chamber assay. Representative Western blot of phospho-ERK1/2 and total ERK1/2 (D) and quantification of Western blot analysis (ratio of phospho-ERK1/2/total ERK1/2) (n = 12). **, p < 0.01; ***, p < 0.005. Results are expressed as mean ± S.E. (error bars).