FIGURE 6.

Effects of dexamethasone (Dex) (1 and 10 μm) and MIF (50 and 250 ng/ml) on MKP-1, ANXA1, and GR mRNA and protein expression in U373 MG cells. A, D, and G, representative gels of RT-PCR products. B, E, and H, quantification of RT-PCR products normalized with the level of 28 S rRNA (n = 5 for MKP-1, n = 3 for ANXA1, n = 4–7 for GR). C, F, and I, representative Western blot of MKP-1, ANXA1, and GR. *, p < 0.05; **, p < 0.01; ***, p < 0.005. A.U., arbitrary units. Results are expressed as mean ± S.E. (error bars).