TABLE 1.

RT-PCR characteristics

Gene and accession number	Primers (forward and reverse)	Initial denaturation	PCR cycles			Final elongation	Number of cycles	Expected size
			Denaturation	Annealing	Elongation
MIF	5′-GAACCGCTCCTACAGCAAGCT-3′	95 °C	94 °C	60 °C	72 °C	72 °C	35 (U373 MG)	121 bp
NM_002415	5′-GCGAAGGTGGAGTTGTTCCA-3′	2 min	15 s	30 s	15 s	2 min	28 (other)
CD74	5′-TGACCAGCGCGACCTTATCT-3′	95 °C	94 °C	52 °C	72 °C	72 °C	38	223 bp
NM_00102515	5′-GTTCTCCAGCTGCAGGTTCT-3′	2 min	15 s	30 s	15 s	2 min
GR	5′-TGATAGCTCTGTTCCAGACTCAAC-3′	95 °C	94 °C	60 °C	72 °C	72 °C	26	269 bp
X03225	5′-AGGGTAGAGTCATTCTCTGCTCAT-3′	2 min	15 s	20 s	20 s	2 min
MKP-1	5′-CCTGACAGCGCGGAATCT-3′	95 °C	94 °C	50 °C	72 °C	72 °C	30	221 bp
NM_004417	5′-TCCTCCACAGGGATGCTC-3′	2 min	15 s	20 s	15 s	2 min
ANXA1	5′-TGAGCCCCTATCCTACCTTCAATC-3′	95 °C	94 °C	68 °C	72 °C	72 °C	20	209 bp
NM_000700.1	5′-TCAAGGTGACCTGTAAGGGCTTC-3′	2 min	15 s	20 s	10 s	2 min
28 S rRNA	5′-GTTCACCCACTAATAGGGAACGTGA-3′	95 °C	94 °C	66 °C	72 °C	72 °C	15	212 bp
U13369	5′-GATTCTGACTTAGAGGCGTTCAGT-3′	2 min	15 s	20 s	10 s	2 min