TABLE 2.

Characterization of U373 MG, Hs 683, U87 MG, LN-18, and T98G glioma cell lines

MIF promoter polymorphisms were determined by PCR and nucleotide sequencing.

Cell line	MIF status					CD74 status	Cortisol secretionc	GR status
	mRNA levela	Intracellular protein	Protein secretionb	−173 G/C single nucleotide polymorphism	Number of CATT repeats
			pg/ml/μg of DNA
U373 MG	Low	−d,e	0	CC	6	−a	no secretion	+a,d,e
Hs 683	High	+d,e	54	GC	6	+a	no secretion	+a,d,e
U87 MG	High	+d	350	GC	6	+a	NDf	+a,d
LN-18	High	+d	90	GG	6	−a	ND	+a,d
T98G	High	+d	145	GC	6	+a	ND	+a,d

^a MIF, CD74, and GR expression was assessed by RT-PCR.

^b MIF, CD74, and GR expression was assessed by ELISA.

^c The endogenous cortisol production was assessed by ECLIA testing.

^d MIF, CD74, and GR expression was assessed by immunohistochemistry.

^e MIF, CD74, and GR expression was assessed by Western Blot.

^f ND, not determined.