FIGURE 6.

Impact of the Atg16L1 WD repeat deletion mutant and T300A mutant on bacterial growth arrest. A and B, wild-type or Atg16L1-Δ/ΔMEFs expressing GFP-LC3 and the indicated constructs were infected with mCherry expressing S. typhimurium (multiplicity of infection = 10) for 1 h, and then analyzed by immunocytochemistry for Atg16L1 (A). The percentage of LC3-positive bacteria was enumerated by fluorescence microscopy. At least 50 bacteria were counted. The average ± S.D. is shown for three independent experiments. Statistical analysis was performed by using Student's t test: **, p < 0.01; NS, not significant. C, wild-type or Atg16L1-Δ/ΔMEFs expressing the indicated constructs were infected with S. typhimurium (multiplicity of infection = 100) for 10 min. After treating with gentamicin for 0, 2, or 6 h, the cells were lysed and serially diluted, and the lysates were plated to determine the number of viable bacteria. Each value represents the mean ± S.D. Statistical analysis was performed by using Student's t test: *, p < 0.05; NS, not significant.