FIGURE 6.

Viability of CDK1-phosphosites rad53 mutants in different stress conditions. Drop test analysis of serial 5-fold dilutions of exponentially growing cell cultures of rad53Δ sml1Δ strains, carrying the indicated rad53 allele under its own promoter on a centromeric plasmid. Isogenic mec1Δ, mad2Δ, and rad53-Δ781 strains were also tested as indicated. A, B, and C, cells were plated on YPD and YPD plus hydroxyurea (HU), methyl methanesulfonate (MMS), benomyl, or calcofluor white at the indicated concentrations. Plates were incubated 3 days at 28 °C (A and C) or 16 °C (B).