FIGURE 3.

IL-15 regulates C/EBPβ and YY1 binding to the mMCP-2 promoter in mouse mast cells. WT and IL-15^−/− BMMCs were analyzed by ChIP using monoclonal Abs to C/EBPβ, YY1, or with an equal amount of unspecific (anti-muscle-specific kinase receptor, Musk) Abs, or isotype-matched control Abs (rabbit IgG), or without Abs, all used as negative control. Immunoprecipitated DNA was analyzed by PCR using mouse mMCP-2-specific primers, which amplify the −189 to +28 bp (Δp189) region. Each panel displays one experiment representative of three that were performed. Input, DNA purified from chromatin that has not been subjected to ChIP. M, 100-bp ladder markers.