FIGURE 4.

Atp8a2 localizes to the disc membranes of outer segments. Approximately 30 μg of crude retina membranes (Retina), isolated ROS, and purified disc membranes (Discs) were resolved by SDS gel electrophoresis. The gels were stained with Coomassie Blue, and Western blots were labeled with the Atp6C11 antibody to Atp8a2, PMc 1D1 antibody to CNGA1 as a plasma membrane-specific marker, and the Rim 3F4 to ABCA4 as a disc-specific marker. The profile of Atp6C11 resembled Rim3F4, indicating that Atp8a2 is localized to disc membranes.