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The binding of 14-3-3 isoforms to negatively charged phospholipid bilayers. A, representative sensorgrams. The L1 sensor chip was loaded with liposomes made of PC:PBPS (1:1); 14-3-3γ (▿), 14-3-3η (□), and 14-3-3ζ (○) proteins (17 μm subunit) were injected (at pH 7.4); and association-dissociation curves were monitored as described under “Experimental Procedures.” B, sensorgrams for the binding of 14-3-3γ to liposomes made of PC:PBPS at pH 7.0 (●), 7.4 (○), 8.0 (▾), and 9.0 (▵).
