TABLE 4.
TH activity measurements
| Proteinsa | Liposomesb | TH activityc | Percentage of control |
|---|---|---|---|
| nmol l-DOPA/min/mg | % | ||
| Control (Ser(P)19-hTH1) | No | 533 ± 52 | 100 |
| Ser(P)19-hTH1 with 14-3-3γ | No | 613 ± 60 | 115 |
| Ser(P)19-hTH1 with 14-3-3ζ | No | 778 ± 80 | 146 |
| Ser(P)19-hTH1 | Yes | 373 ± 50 | 70 |
| Ser(P)19-hTH1 with 14-3-3γ | Yes | 544 ± 10 | 102 |
| Ser(P)19-hTH1 with 14-3-3ζ | Yes | 480 ± 90 | 90 |
a The concentration of Ser(P)19-hTH1 and 14-3-3 proteins was 0.05 μm and 1.8 μm subunit, respectively.
b Large unilamellar vesicles made of PC:PBPS:SDPS:DPPC, 20 μm final lipid concentration.
c The results represent the mean ± S.D. of three measurements.