Table 3.

In situ hybridization showing mRNA levels in control and transgenic R6/2 mice

ISH probe	Striatum		Cortex
	Control	Transgenic	Control	Transgenic
mGluR1
4 weeks	245.5  ±  12.1	187.9  ±  9.1*	100  ±  6.1	90.9  ±  6.1
8 weeks	261.5  ±  30.7	126.9  ±  7.7*	100  ±  11.5	80.8  ±  3.8
12 weeks	278.4  ±  35.3	143.1  ±  9.8*	100  ±  5.9	72.5  ±  5.8*
mGluR2
4 weeks	28.8  ±  1.5	27.2  ±  1.5	100  ±  3.0	81.8  ±  7.6
8 weeks	15.2  ±  1.5	6.6  ±  1.5	100  ±  7.7	56.4  ±  5.1†
12 weeks	28.8  ±  1.5	36.4  ±  1.5	100  ±  5.3	54.9  ±  3.5‡
mGluR3
4 weeks	77.2  ±  4.7	77.6  ±  5.6	100  ±  6.3	84.3  ±  5.5
8 weeks	86.2  ±  6.2	66.7  ±  4.6	100  ±  7.7	76.9  ±  7.7
12 weeks	59.6  ±  5.6	41.4  ±  2.5	100  ±  5.6	58.6  ±  2.5‡
mGluR5
4 weeks	211.3  ±  11.3	196.9  ±  6.2	100  ±  5.1	113.4  ±  3.1
12 weeks	236.5  ±  22.4	197.9  ±  14.6	100  ±  8.3	114.6  ±  10.3
NMDA-R1
4 weeks	77.7  ±  3.6	67.4  ±  3.6	100  ±  5.2	93.4  ±  5.7
8 weeks	76.6  ±  5.7	55.3  ±  4.5	100  ±  6.1	90.2  ±  8.6
12 weeks	77.1  ±  8.7	80.8  ±  6.6	100  ±  8.9	96.9  ±  6.8
D1 dopamine
4 weeks	100  ±  7.7	53.8  ±  2.8†	–	–
8 weeks	100  ±  8.7	60.5  ±  6.3*	–	–
12 weeks	100  ±  4.3	37.7  ±  1.7‡	–	–
D2 dopamine
4 weeks	100  ±  3.1	82.3  ±  5.1	–	–
8 weeks	100  ±  8.1	39.0  ±  4.2‡	–	–
β-actin
4 weeks	94.2  ±  1.9	89.6  ±  1.9	100  ±  1.9	96.1  ±  1.9
12 weeks	94.5  ±  7.4	86.8  ±  6.1	100  ±  4.3	101.5  ±  5.2

Numbers represent film optical density (OD) expressed as a percentage of signal in the cortex of control mice (mean ± SEM, n = 6–8 mice per group). For D₁ and D₂ dopamine receptor mRNA, numbers represent film OD expressed as a percentage of signal in striatum of control mice. 

^*P < 0.01;  

^†P < 0.001;  

^‡P < 0.0001.