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. 2009 Sep 21;284(47):32869–32880. doi: 10.1074/jbc.M109.022582

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FIGURE 4. — D-AKAP2 forms protein complexes with Rab4 and Rab11. A, HEK293 cells were co-transfected with FLAG-tagged D-AKAP2, vector, or a truncation containing the two RGS domains (residues 1–513 (last two lanes)) together with GFP-tagged Rab proteins or mutants. Samples from the lysate and from the elution of the immunoprecipitation (IP) were immunoblotted (IB) with antibodies against either FLAG or GFP. B, free GST or GST-Rab4 protein loaded with GDP or GTPγS was incubated with a HEK293 lysate containing endogenous and overexpressed tagless D-AKAP2, pulled down with glutathione resin, and immunoblotted for co-precipitated D-AKAP2.