FIGURE 1.
SPS1/SelD knockdown does not reduce the level of SPS2 mRNA. A, level of SPS1/SelD and SPS2 mRNA was measured by real time reverse transcription-PCR and normalized to that of rp49. The relative mRNA levels of SPS1/SelD and SPS2 in SPS1/SelD knockdown cells (closed bars) were calculated by comparing the normalized value to those in GFP dsRNA-treated cells (open bars), the value of which was set to 100%. B, level of SPS1/SelD was determined by Western blotting using anti-SPS1/SelD antibody. α-Tubulin was used as a loading control.