TABLE 2.
Inhibition of megamitochondrial formation
| dsRNA including SPS1/SelD dsRNA | No. of total cells counted | Frequency of MMta formation (mean ± S.D., %) | Inhibition rateb % | Gene ontology (biological process) |
|---|---|---|---|---|
| GFP | 158 | 77.2 ± 1.8 | 0 | —c |
| Pepck | 154 | 75.3 ± 1.3 | 2.5 | Gluconeogenesis |
| Adk3 | 170 | 67.1 ± 1.5 | 13.2 | ADP biosynthetic process |
| CG8745 | 181 | 73.5 ± 2.1 | 4.8 | Amino acid metabolism |
| CG31075 | 160 | 68.2 ± 1.9 | 11.7 | Pyruvate metabolic process |
| GS1 | 140 | 7.9 ± 0.2 | 89.8 | Glutamine metabolic process |
a MMt is megamitochondria.
b Inhibition rate (%) was calculated as 100 × (1 − FTG/FCG), where FTG designates the frequency of megamitochondria formation by the double knockdown of the test gene with SPS1/SelD and FCG the frequency of megamitochondria formation by the double knockdown of control genes (SPS1/SelD and GFP).
c Control (GFP).