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Electrophilic affibody improves stability and has high specificity at the mammalian cell surface. COS7 cells expressing ZSPA-TM N6C or N6C F13E were labeled with biotinylated affibody D36C with or without EBA. Affibody binding was detected with streptavidin-Alexa Fluor 555 (red), and this staining is overlaid with cyan fluorescent protein co-transfection marker (blue) and bright field images. Scale bar, 20 μm.
