FIGURE 4.

PPARγ-regulated ABCA1 mediates IL10-indcued cholesterol efflux in macrophages. A, transcripts for ABCA1 were detected by real-time PCR either in resting macrophages treated with AcLDL or with AcLDL plus IL10, or in lipid-laden macrophages treated with medium or with IL10 for 8 or 24 h, as indicated. The results are representative of three independent experiments, and values are expressed as the -fold change in abundance ± S.E. B–E, BMDMs were incubated for 24 h with the reagents indicated. Expression of ABCA1 protein was examined by immunoblot analysis using rabbit anti-ABCA1 antibody. Representative results from three experiments are shown in B and D. The densities of bands corresponding to ABCA1 were quantitated using an image densitometer. The data shown are band densities in arbitrary optical density units from three repeated experiments (C and E). Ctl, control.