FIGURE 3.

The AD2 activation domain of E47 is phosphorylated in the presence of MLK2. A, scheme showing the different domains in E47. AD1 and AD2, activating domains. Ordered deletions used to delimit the main phosphorylation region of E47 are depicted. The sequence of a putative MLK docking site is aligned with those defined in several MLK substrates. Chemically identical (*), highly conserved (:), or less conserved (.) amino acids are indicated. Chemical characteristics of conserved amino acids are indicated in red (acid), blue (basic), magenta (polar), and green (hydrophobic). B, analysis of E47 regions phosphorylated by MLK2 in vivo. TAP-tagged E47 constructs were transfected in HEK293T in the absence or presence of MLK2 and E47 phosphorylation was assessed by Western blot.