Table IV.
Results of the quantitative PCR validation of differentially expressed genes between PCV2-inoculated and control pigs and comparison with microarray gene expression data.
| Genes | Quantitative PCR |
Microarrays |
||||||
|---|---|---|---|---|---|---|---|---|
| FC | Log2 FC | S.E. | p value | Probes | FC | Log2 FC | p value | |
| Mediastinal lymph node | ||||||||
| IFNG | 3.63 | 1.86 | 0.38 | 0.0001 | Ssc.4093.1.A1_at | 2.91 | 1.54 | 0.0086 |
| IgG | 2.93 | 1.55 | 0.44 | 0.0026 | Ssc.11070.1.S1_at | 2.19 | 1.13 | 0.0981 |
| Ssc.13778.1.S1_at | 2.22 | 1.15 | 0.0236 | |||||
| LGALS3 | −3.70 | −1.89 | 0.53 | 0.0023 | Ssc.17815.1.S1_at | −2.86 | −1.50 | 0.0086 |
| Mx1 | 2.62 | 1.39 | 0.66 | 0.0501 | Ssc.221.1.S1_at | 2.67 | 1.41 | 0.0699 |
| OAS1 | 3.92 | 1.97 | 0.69 | 0.0103 | Ssc.1031.1.S1_at | 3.53 | 1.82 | 0.0887 |
| Blood (7 dpi) | ||||||||
| OAS1 | 11.96 | 3.58 | 1.07 | 0.0156 | Ssc.1031.1.S1_at | 13.69 | 3.78 | 0.0039 |
FC: fold change, S.E.: standard error.