Table 3. Bacterial strains, plasmids and oligonucleotides used in this study.
| Strains, vectors and primers | Descriptiona | Source or Reference |
| Strains | ||
| P. aeruginosa | ||
| MPA | mutS::ISlacZA/hah-TcR, MPAO1 derivative | [53] |
| MPA-T1 | TcR, MPA carrying a mucAT1 allele | This study |
| MPA-T2 | TcR, MPA carrying a mucAT2 allele | This study |
| MPA-T3 | TcR, MPA carrying a mucAT3 allele | This study |
| E. coli | ||
| XL1-Blue | host strain for DNA manipulation | [60] |
| SY327 λpir | RfR; recipient to propagate pKNG-101 derivatives | [61] |
| SM10 λpir | Recipient for conjugal transfer of pKNG-101 derivatives | [61] |
| Vectors | ||
| pGEM-T Easy | ApR; PCR product cloning vector | Promega |
| pGEM-mucAT1 | ApR; P. aeruginosa mucAT1 cloned in pGEM-T Easy | This study |
| pGEM-mucAT2 | ApR; P. aeruginosa mucAT2 cloned in pGEM-T Easy | This study |
| pGEM-mucAT3 | ApR; P. aeruginosa mucAT3 cloned in pGEM-T Easy | This study |
| pKNG101 | SmR, suicide delivery plasmid containing sacB gene (SucS) | [55] |
| pKNG-mucAT1 | SmR, SucS, pKNG101 carrying mucAT1 | This study |
| pKNG-mucAT2 | SmR, SucS, pKNG101 carrying mucAT2 | This study |
| pKNG-mucAT3 | SmR, SucS, pKNG101 carrying mucAT3 | This study |
| Primers | ||
| MucPA-F | 5′-GAAGCCTGACACAGCGGCAAATGC-3′ | [21] |
| MucPA-R | 5′-CCTCAGCGGTTTTCCAGGCTGGCTGC-3′ | [21] |
| MucBamHI-F | 5′-TATGGATCCTGAAGCAATCGACAAAGCTC-3′ | This study |
| MucXbaI-R | 5′-TTATCTAGAAGCTGGGAGGGATCGAACTT-3′ | This study |
| MucT1-F | 5′-GCGAAGAGCAAGGTGCGCCGCAGG-3′ | This study |
| MucT1-R | 5′-CCTGCGGCGCACCTTGCTCTTCGC-3′ | This study |
| MucT2-F | 5′-GCAAGGTGCCCCCCAGGTGATCACCAACTCCTC-3′ | This study |
| MucT2-R | 5′-CTGGGGGGCACCTTGCTCTTCGCTGTAGCCGG-3′ | This study |
| MucT3-F | 5′-AGCAGGGGGCCCCCCAGGTGATCA-3′ | This study |
| MucT3-R | 5′-TGATCACCTGGGGGGCCCCCTGCT-3′ | This study |
a
Resistance markers: Tc, tetracycline; Rf, rifampin; Sm, streptomycin; Ap, ampicillin; Suc, sucrose.