Table 1.

Validation of DSI Tractography.

	Organ	Known Anatomical Features
Wedeen et al [33]	Brain	Crossing nerve tracts (optic chiasm, brainstem and others)
Gilbert et al [36]	Tongue	Core of crossing fiber tracts
Sosnovik et al [27]	Myocardium	Array of crossing helical myofibers
	Organ	Validation Technique
Lin et al [37]	Optic tracts	Manganese-enhanced MRI
Schmahmann et al [61]	Brain	Autoradiography
Gaige et al [62]	Tongue	Two-photon microscopy
Sosnovik et al [27]	Myocardium	Histology

The technique has been validated in several organ systems by resolving known anatomical features and also by direct comparison with a second imaging modality. A partial list of the performed validation studies is provided.