FIGURE 8.

CBP shRNA inhibits TGF-β1-induced Smad3 acetylation and EBV-lytic cycle. A, Mutu-I, Kem-I, and Sav-I cells infected with CBP shRNA lentiviral particles or control particles were stimulated or not with TGF-β1 (2 ng/ml) for 17 h. The cell lysates were immunoprecipitated (IP) with anti-Smad3 antibody, and acetylated Smad3 was detected by immunoblot with anti-acetyllysine antibody. Simultaneously, equal amounts of protein from total lysate were separated by SDS-PAGE and analyzed by Western blotting with antibodies to ZEBRA, EA, VCA, phospho-Smad3, Smad3, phospho-Akt, Akt, and tubulin. B, 3 μg of total RNA from Mutu-I, Kem-I, and Sav-I cells infected with CBP shRNA lentiviral particles or control particles stimulated or not with TGF-β1 (2 ng/ml) for 17 h were reverse transcribed. cDNA coding for ZEBRA was then analyzed by PCR; cDNA of hypoxanthine-guanine phosphoribosyltransferase (HPRT) was used as an internal control.