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Comparison of E. coli and yeast ValRS. Although the catalytic cores of E. coli and yeast ValRSs are significantly homologous to each other, the yeast cytoplasmic (cyt) protein contains an N-terminal domain of ∼97 residues that is absent from its E. coli counterpart. The polypeptide sequence of the mitochondrial (mit) precursor form of yeast ValRS is essentially identical to that of its cytoplasmic isoform except for a mitochondrial targeting signal attached at its N terminus.
