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In vivo activity of single catalytic-domain mutants. A, yield of active vtPA in SF100 ΔdegP ΔdsbC (gray bars) or SF100 ΔdegP dsbA::kan (black bars) cells was determined as described under “Experimental Procedures.” B, PhoA activity in E. coli MC1000 dsbA::kan (white bars) and MC1000 dsbB::kan (black bars). The alkaline phosphatase activity in the parental isogenic strain MC1000 is shown by the gray bar.
