TABLE 1.

In vitro activities of purified enzymes

Enzyme	RNAse refoldinga		Insulin reduction
	μm/min/μm Enzyme	% Activity	×10−3 ΔA650/min2	% Activity
DsbC	0.076 ± 0.007	100	11.1 ± 4.8	100
mDsbC-mDsbC	0.064 ± 0.008	84	8.63 ± 3.0	78
DsbC[C98A]	0.004 ± 0.001	5	0.04 ± 0.01	0
mDsbC-mDsbC[C98A]	0.052 ± 0.008	68	3.37 ± 1.0	30
DsbC[C98A/C101A]	0.001 ± 0.001	1	0.04 ± 0.01	0
mDsbC-mDsbC[C98A/C101A]	0.038 ± 0.005	50	3.19 ± 0.12	29
DsbC[C101A]	0.081 ± 0.009	107	12.88 ± 2.6	116
mDsbC-mDsbC[C101A]	0.079 ± 0.005	104	8.57 ± 2.0	77
mDsbC-dim	0.038 ± 0.005	50	1.95 ± 0.61	18
mDsbC[H45D]-dim[D53H]	0.024 ± 0.005	32	1.67 ± 0.13	15
mDsbC[H45D]-dim[H45D]	0.009 ± 0.002	12	Ndb	Nd

^a The activities were determined from a plot of isomerization velocity against enzyme concentrations.

^b Nd, not determined.