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Phosphorylated Thr-187 is the major Pin1-binding site on p27Kip1. A, fitted binding curves for Pin1 recombinant protein bound to three phosphorylated peptides derived from p27Kip1. B, phosphorylated peptide sequences and Kd and S.D. values calculated from the binding curve shown in A, indicating the affinity of Pin1 for phosphorylated peptides derived from p27Kip1.
