Table 1.
Ectopic expression of OmpT in place of OmpU diminishes V. cholerae toxT transcription and CT/TCP expression
| Relevant genotype* | Plasmid† | Agg | TCP‡ | CT§ | toxT∷lacZ¶ | % HEp-2‖ adherence |
|---|---|---|---|---|---|---|
| Wild type (toxR+) | pWSK30 | + | 8.4 × 104 | 1010 | 96 ± 5 | 9.1 (4.3) |
| ΔtoxR | pWSK30 | − | 0 | <1 | 15 ± 1 | 3.4 (2.7) |
| ΔompU (toxR+) | pWSK30 | + | 8.6 × 104 | 880 | 115 ± 6 | 10.0 (4.3) |
| ΔompU (toxR+) | Up-U | + | 4.4 × 104 | 935 | 111 ± 5 | 2.5 (2.9) |
| ΔompU (toxR+) | Up-T | − | 7.7 × 102 | 10 | 62 ± 6 | 4.3 (6.1) |
*
Actual strains used (see Materials and Methods) O395, KKV61, KKV780, O395 toxT∷lacZ, KKV526, and KKV1089.
†
Plasmids used (see Materials and Methods): Up-U, pKEK253; and Up-T, pKEK257.
‡
CTXΦKan transductants/ml/OD600.
§
ng/ml/OD600.
¶
Miller units based on triplicate samples.
‖
% adherent bacteria of total added; shown in parentheses are results of a second independent experiment.