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CsA inhibits secretion of apoE from HMDM and CHO-apoE cells. Human macrophages (A–C) were incubated with indicated concentrations of CsA for 3 h, and CHO-apoE cells (D and E) were treated with 5 μm CsA for 1 h. Secreted apoE (A and D), cellular apoE levels (B and E), and apoE mRNA levels (C) were determined by ELISA, Western analysis, and real time PCR, respectively. *, p < 0.05 relative to control; AU, absorbance units.
