FIGURE 6.

CsA inhibits apoE secretion via inactivation of PP2B. HMDMs were incubated with indicated concentrations of FK506 for 3 h, and secreted apoE was measured by ELISA (A), cell apoE measured by Western blot (B), and PP2B activity in HMDM cell lysates after treatment with 10 μm CsA and 25 μm FK506 (C). D, apoE secreted after treatment with indicated concentrations of okadaic acid after 1 h. E and F, secreted apoE and cell apoE, respectively, by Western blot after treatment with 35 μm of PP2B autoinhibitory peptide. *, p < 0.05 relative to control (Ctrl); AU, absorbance units.