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CsA inhibits basal and apoA-I-stimulated apoE secretion from macrophages derived from ABCA1−/− mice. BMDM from wild type (wt) and ABCA1−/− mice were incubated with 10 μm CsA for 1 h and then washed and exposed to CsA with or without 10 μg/ml apoA-I (AI). After 3 h, apoA-I-specific cholesterol efflux (A) and secreted apoE (B) were determined as described under “Experimental Procedures.” *, p < 0.05 relative to control (Ctrl); #, p < 0.05 relative to apoA-I; AU, absorbance units.
