TABLE 2.
Structural statistics of hepcidin determined by NMR
| NMR-derived distance restraints | High temperature | Low temperature |
|---|---|---|
| Unambiguous NOEs | 186 | 170 |
| Ambiguous NOEs | 0 | 0 |
| Sequential | 160 | 158 |
| Medium (2–4) | 3 | 5 |
| Long range (>4) | 23 | 7 |
| Total NOEs | 186 | 170 |
| Hydrogen bonds | 5 | 5 |
| Root mean square relative to mean (6-24)a | ||
| Backbone atoms | 0.39 ± 0.25 | 0.68 ± 0.27 |
| All heavy atoms | 0.86 ± 0.27 | 1.21 ± 0.24 |
| Ramachandran statistics (%)b | ||
| Most favored | 63.8 | 68.8 |
| Additionally allowed | 36.2 | 26.2 |
| Generously allowed | 0 | 5.0 |
| Disallowed | 0 | 0 |
| CYANA target function | 1.06 | 2.04 |
| Violated NOE restraints | 0 | 0 |