TABLE 3.
Summary of hydrodynamic and kinetic parameters after the addition of each component of the MLL1 core complex
| Protein complex | sa | s20,w | f/f0 | Calculated mass | Theoretical mass | k1b |
|---|---|---|---|---|---|---|
| kDa | kDa | h−1 | ||||
| MLL3745 | 1.7 ± 0.01 | 1.8 | 1.7 | 26.1 | 26.1 | 0.003 ± 0.0003 |
| MLL3745-WDR5 | 2.9 ± 0.02 | 3.1 | 1.7 | 62.0 | 62.6 | 0.003 ± 0.0004 |
| MLL3745-WDR5-RbBP5 | 3.7 ± 0.04 | 3.9 | 2.2 | 110.0 | 121.7 | 0.007 ± 0.0004 |
| MLL3745-WDR5-RbBP5-Ash2L | 5.0 ± 0.02 | 5.4 | 2.0 | 180.0 | 179.0 | 0.93 ± 0.07 |
| MLL3745-WDR5-RbBP5-Ash2L-(DPY-30)2 | 5.3 ± 0.01 | 5.7 | 2.0 | 198.0 | 201.4 | 1.77 ± 0.11 |
a Experimental sedimentation coefficient determined at 10 °C (±S.E. from two or three independent experiments).
b Pseudo-first-order rate constant k1 (±S.E.) determined from fitting Equation 1 to the disappearance of the relative intensity of the unmodified histone H3 peptide in single turnover progress curves.