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. 2009 Jul 8;284(36):24289–24296. doi: 10.1074/jbc.M109.007286

FIGURE 3.

FIGURE 3.

Loss of binding and signal transduction of IL-13, but not IL-4 in Val-35 → Ala, Leu-38 → Ala, and Val-42 → Ala of IL-13Rα1. A, expression of the wild type, D1-deleted type, and the indicated mutated types of IL-13Rα1 on the surface of stable DND-39/Gϵ transfectants as estimated by flow cytometry is shown using anti-FLAG Ab. The shaded and open areas represent the count with or without the first Ab, respectively. B, binding of 125I-labeled IL-13 (closed circles) or IL-4 (closed triangles) to DND-39/Gϵ cells expressing the wild type, D1-deleted type, and the indicated mutated types of IL-13Rα1 is depicted. C and D, activation of STAT6 (C) and Tyk2 (D) using DND-39/Gϵ cells expressing the wild type, D1-deleted type, and the indicated mutated types of IL-13Rα1 is shown. The cells were stimulated with either 0.01 ng/ml of IL-13 (C) or 10 ng/ml of IL-4 or IL-13 (D) for 10 min.