FIGURE 5.

Importance of Lys-76, Lys-77, and Ile-78 in IL-13Rα1 for binding to IL-13. A, schematic models of two internal deleted mutants of IL-13Rα1 are depicted. The numbers represent the deleted amino acid numbers. The wild type or the indicated internal deleted mutated type of IL-13Rα1 (B and C) was transfected into HEK 293T cells. Expression of IL-13Rα1 (B) on the surface of HEK 293T cells by flow cytometry using anti-FLAG Ab is shown. The shaded and open areas represent the expression of the indicated transfectant or mock transfectant, respectively. Binding of ¹²⁵I-labeled IL-13 (C) to HEK 293T cells is depicted.