FIGURE 1.

The basic domain of JDP2 is the minimal region to interact with PR. A, schematic of the nuclear receptor DBD showing the Gly-Met (GM) boundary distinguishing the core DBD from the CTE. Open circles represent amino acids, and light gray circles represent helical regions. Coordinated zinc (Zn) and cysteine (C) residues are labeled. Amino acids comprising the proximal box (P box) have lines drawn through them, whereas residues comprising the dimerization box (D box) are darker gray. B, constructs of PR and JDP2 used in protein-protein interaction assays including PR DBD-CTE₆₄₁, GST·JDP2, GST·JDP2 amino terminus (N) +basic domain, GST·JDP2 bZIP, GST·JDP2 basic domain, and GST·JDP2 ZIP. C, PR DBD-CTE₆₄₁ was incubated with free GST or the different GST·JDP2 fusion proteins immobilized to glutathione-Sepharose resins. After washing resins, bound protein was eluted and detected by immunoblot assay with a PR DBD-specific antibody along with input PR DBD-CTE₆₄₁.