TABLE 2.
Enzyme | Substrate | Product | Km | kcat | kcat//Km |
---|---|---|---|---|---|
μm | pmol/min/pmol P450 | mm−1s−1 | |||
P450 2D6 | (R)-Reticuline | Corytuberine | 2.7 ± 0.37 | 0.015 ± 0.001 | 0.09 |
Pallidine | 1.8 ± 0.26 | 0.045 ± 0.002 | 0.42 | ||
Salutaridine | 2.5 ± 0.21 | 0.011 ± 0.001 | 0.08 | ||
Isoboldine | 1.9 ± 0.36 | 0.390 ± 0.026 | 3.42 | ||
Thebaine | Oripavine | 48 ± 9.6 | 4.55 ± 0.54 | 1.60 | |
Codeine | Morphine | 250a | 14a | 0.93 | |
190b | 6.4b | 0.56 | |||
P450 3A4 with cytochrome b5 | (R)-Reticuline | Corytuberine | 384 ± 40 | 1.3 ± 0.1 | 0.06 |
Pallidine | 474 ± 30 | 14.7 ± 0.3 | 0.52 | ||
Salutaridine | 1961 ± 181 | 12.3 ± 0.5 | 0.12 | ||
Isoboldine | 4860 ± 860 | 158 ± 17 | 0.54 | ||
P450 3A4 | (R)-Reticuline | Corytuberine | 534 ± 66 | 0.16 ± 0.14 | 0.005 |
Pallidine | 490 ± 38 | 2.97 ± 0.14 | 0.101 | ||
Salutaridine | 993 ± 220 | 2.20 ± 0.36 | 0.037 | ||
Isoboldine | 577 ± 130 | 9.37 ± 1.34 | 0.271 |