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. 2009 Jun 30;284(36):24432–24442. doi: 10.1074/jbc.M109.033373

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Detection of recombinant CYP719B1 by SDS-PAGE and reduced CO difference spectrum. A, SDS-PAGE of recombinant CYP719B1 and CPR. Recombinant CYP719B1 and CPR produced in Sf9 insect cell cultures were resolved by SDS-PAGE and visualized with Coomassie Brilliant Blue G-250. Lane a, molecular mass markers: 97.4 kDa, phosphorylase b; 66.2, bovine serum albumin; 45, ovalbumin; 31, carbonic anhydrase; 21.5, soybean trypsin inhibitor; lane b, CYP719B1 expressed in Sf9 cells; lane c, CYP719B1 and CPR co-expressed in Sf9 cells; lane d, STR of Psychotria ipecacuanha expressed in Sf9 cells (as control, Nomura et al., unpublished); lane e, CPR expressed in Sf9 cells. Arrow indicates position of CYP719B1 protein; asterisk indicates position of CPR protein. B, reduced CO difference spectrum obtained with microsomes isolated from Sf9 cells co-infected with baculoviruses containing CYP719B1 and E. californica CPR (38).