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. 2009 Jun 30;284(36):24432–24442. doi: 10.1074/jbc.M109.033373

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Mass spectrometric analyses of reaction products produced by CYP719B1 from (R)-reticuline and (R)-norreticuline. Selected ion monitoring (m/z 330 for (R)-reticuline, m/z 316 for (R)-norreticuline, m/z 328 for salutaridine and m/z 314 for norsalutaridine is shown. A, (R)-reticuline standard; B, salutaridine standard; C, product of the enzyme assay containing (R)-reticuline and recombinant CYP719B1 and CPR; D, (R)-norreticuline standard; E, product of the enzyme assay containing (R)-norreticuline and recombinant CYP719B1 and CPR. Assay and MS conditions are given under “Experimental Procedures.”