TABLE 3.

Acceptor specificity of OatWY

Acceptora	Activity
	%
Polysaccharide [Glc-(α1→4)-Sia]n	100.0 ± 0.3
Disaccharide [Glc-(α1→4)-Sia]	19.2 ± 0.2
Colominic acid [α(2→8)Sia]n	2.1 ± 0.2
Neu5Ac	0.3 ± 0.2
CMP-Neu5Ac	0.3 ± 0.1
Glucose	0.0 ± 0.2
S-(2-oxopropyl)-CoAb	2.4 ± 0.1
(−)-Control (water)	0.7 ± 0.1

^a Concentrations of acceptors were adjusted to 5 mm sialic acid equivalent to that described under “Experimental Procedures.”

^b Instead of acetyl-CoA, the S-(2-oxopropyl)-CoA was added to the assay mixture in the presence of the natural acceptor ([Glc-(α1→4)-Sia]_n).