FIGURE 6.

Molecular interactions of LOX with fibrillin-1 and tropoelastin. A, solid phase binding assays showing soluble biotinylated LOX binding to immobilized fibrillin-1. i and ii, LOX binds to the N-terminal fibrillin-1 fragment PF1 and shows reduced binding to PF1 deletion fragments but no binding to Ex5–7. iii, binding curve showing soluble biotinylated LOX binding to immobilized PF1. One representative experiment is shown. Data are shown with the negative (biotinylated LOX only) control subtracted. Results are shown as the mean ± S.E. of triplicate values. B, solid phase binding curve showing soluble biotinylated LOX binding to immobilized tropoelastin. One representative experiment is shown. Data are shown with the negative (biotinylated LOX only) control subtracted. Results are shown as the mean ± S.E. of triplicate values.