FIGURE 3.

Relative amounts of mRNAs encoding SREBP pathway components and SREBP targets in livers from C57BL/6J and SJL/J under fed and fasted conditions. Male C57BL/6J and SJL/J mice (9 weeks of age) were fed a chow diet ad libitum or fasted for 24 h. Total RNA from 6 livers from each strain were pooled and quantified by real-time PCR as described under “Experimental Procedures.” Each value represents the amount of mRNA relative to that in the liver of fed C57BL/6J, which is arbitrarily defined as 1.0. ACS, acetyl-CoA synthetase; G6PD, glucose-6-phosphate dehydrogenase; ACC-1, acetyl-CoA carboxylase-1; FAS, fatty acid synthase; LCE, long-chain fatty acyl elongase; SCD-1, stearoyl-CoA desaturase-1; GPAT, glycerol-3-phosphate acyltransferase; FDP Syn, farnesyl diphosphate synthase; LDLR, low density lipoprotein receptor. Similar results were obtained in two other independent experiments.