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Enhanced PKD signaling at the NHERF scaffold. MDCK cells overexpressing DKAR (circles) or DKAR-PDZ (diamonds) in the absence (open symbols) or presence (closed symbols) of overexpressed NHERF-1 were treated with 200 nm PDBu, and the ratio of cyan emission to yellow emission was monitored with time. FRET ratios were normalized and plotted over time. The data represent the averages ± S.E. from at least three independent experiments.
