FIGURE 5.

PKD signaling at NHERF-1 is distinct from PKD signaling at the plasma membrane. A, MDCK cells overexpressing DKAR-PDZ and NHERF-1 (closed diamonds), MyrPalmDKAR alone (open squares), or MyrPalmDKAR and NHERF-1 (closed squares) were stimulated with 200 nm PDBu, and the ratio of cyan emission to yellow emission was monitored with time. B, PKD activity in the stimulated cells in A was inhibited following addition of the PKD inhibitor Gö 6976 (500 nm). FRET ratios were normalized and plotted over time. The data represent the averages ± S.E. from at least three independent experiments.