FIGURE 7.

NHERF-1 depletion reduces PKD substrate phosphorylation of select substrates. HeLa cells treated with control siRNA (si control) or NHERF-1 siRNA (si NHERF-1) were stimulated for the indicated times with 10 μm histamine. The cell lysates were analyzed by Western blotting using an antibody made against a phosphorylated PKD substrate consensus sequence (α-phospho-PKD substrate). A PKD substrate that co-migrated with the 100-kDa marker displayed reduced phosphorylation in the NHERF-1 knockdown cells (marked by an arrow). The graph depicts the level of phosphorylation of the 100-kDa substrate normalized to the 0-min time point of the siRNA control-treated cells. Actin was used as a loading control. The data represent the averages of two independent experiments. The error bars represent the ranges of the responses.