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. 2009 Oct 1;28(22):3623–3632. doi: 10.1038/emboj.2009.287

Figure 3.

Figure 3

A key role for a talin/membrane interaction in integrin activation. (A) GFP-talin1 F1–F2–F3 wild type (F1–F2–F3) or F1–F2–F3 with various mutations in the F2 domain were transfected into αIIbβ3-expressing CHO cells. Activated integrins were detected with PAC1 antibody and analysed by FACS 24 h after transfection. Integrin activity was normalized against GFP-F1–F2–F3 wt-transfected cells. Error bars represent standard errors of three independent experiments. 4E corresponds to all four membrane orientation patch (MOP) residues mutated to glutamate, and 4A corresponds to all four mutated to alanine. (B) As in (A), but with GFP-talin1 F3 wt or F1–F2–F3 wt. Error bars (barely visible because of small size) represent standard errors of two independent experiments. F3 caused a statistically significant increase in integrin activation (*) of P=0.0388 by one tail test. (C) As in (A) and (B), but GFP-talin1 F3 wt, F1–F2-F3 wt, or F1–F2–F3 mutants were transfected into CHO cells expressing a chimaeric integrin containing the intracellular domains of α5β1A, as described in the text. Error bars represent standard errors of four independent experiments.